Bubaline tissues-based extracellular matrix scaffolds for tissue regeneration: current use and future prospectives

Naveen Kumar; Vineet Kumar; Anil Kumar Gangwar; Sonal  Saxena; Pawan Diwan Singh  Raghuvansi; Dayamon D.  Mathew; Sameer Shrivastava; Ashwathy Gopinathan

doi:10.55092/bm20230008

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Bubaline tissues-based extracellular matrix scaffolds for tissue regeneration: current use and future prospectives

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Naveen Kumar¹^,∗, Vineet Kumar², Anil Kumar Gangwar³, Sonal Saxena⁴, Pawan Diwan Singh Raghuvansi⁵, Dayamon D. Mathew⁶, Sameer Shrivastava ⁷, Ashwathy Gopinathan⁸

¹ Veterinary Clinical Complex, Apollo College of Veterinary Medicine, Agra Road, Jaipur- 302031, Rajasthan, India

² Department of Veterinary Surgery and Radiology, College of Veterinary & Animal Sciences, Kishanganj-855107, Bihar Animal Science University, Bihar, India

³ Department of Veterinary Surgery and Radiology, Former Registrar and Deputy Director Research, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya-224 229, Uttar Pradesh, India

⁴ Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute [Deemed University], Izatnagar-243122, Uttar Pradesh, India

⁵ Department of Veterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry, NDVSU, Mhow-453446, Indore, Madya Pradesh, India

⁶ Department of Veterinary Surgery and Radiology, Faculty of Veterinary and Animal Sciences Banaras Hindu University, Rajiv Gandhi South Campus, Barkachha, Mirzapur- 321001, Uttar Pradesh, India

⁷ Bioassays & Biosensor Laboratory, Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute [Deemed University], Izatnagar-243122, Uttar Pradesh, India

⁸ Division of Surgery, ICAR-Indian Veterinary Research Institute [Deemed University], Izatnagar-243122, Uttar Pradesh, India

* naveen.ivri1961@gmail.com

Volume
Volume 1 Issue 1, 2023
Citation
Kumar N, Kumar V, Gangwar AK, Saxena S, Raghuvansi PDS, et al. Bubaline tissues-based extracellular matrix scaffolds for tissue regeneration: current use and future prospectives. Biofunct. Mater. 2023(1):0008, https://doi.org/10.55092/bm20230008.
DOI
10.55092/bm20230008
Copyright
Copyright2023 by the authors. Published by ELSP.
Special Issue
Biofunctional Materials for Tissue Regeneration

Abstract

Most of the collagen scaffolds used in tissue engineering belongs to porcine or bovine origin. Bovines are prone to different zoonotic diseases like bovine spongiform encephalopathy or mad cow disease, transmissible spongiform encephalopathy and foot and mouth disease. The collagen scaffolds developed from porcine origin has little clinical acceptance in our country on cultural and religious grounds. More over the slaughtering of cattle is illegal/ban in most of the part of India. So, we must look the other sources of the collagen. The collagen scaffolds developed from bubaline (buffalo origin) allow greater clinical acceptance on cultural and religious grounds in addition to presenting a low risk with respect to viral pathogens and prions. Therefore, in the present study we developed collagen scaffolds from bubaline (buffalo origin) species. The protocols for decellularization of collagen scaffolds from buffalo aorta, diaphragm, rumen, reticulum, omasum, skin, gall bladder and pericardium have been optimized. The developed acellular collagen matrices were preclinically tested in laboratory animals for their biocompatibility. Suitably found matrices were treated clinically in different species of animals for reconstructive surgery. We succeeded in preservation of the complex composition and three-dimensional ultrastructure of the ECM. No methods of decellularization resulted in disruption of the architecture and loss of surface structure and composition. The acellular ECM scaffolds can create a favorable regenerative microenvironment, promote tissue-specific remodeling, and act as an inductive template for the repair and functional reconstruction. In the current review, we will provide a critical overview of the structure and function of various types of extracellular matrix derived from bubaline (buffalo origin), the construction of three-dimensional extracellular matrix scaffolds, and their tissue engineering applications, with a focus on translation of these novel tissue engineered products to the human applications. We will also present an outlook on future perspectives of the extracellular matrix derived from bubaline (buffalo origin), in tissue engineering and regenerative medicine.

Keywords

Bubaline tissues; extracellular matrix; scaffolds; tissue regeneration; preclinical evaluation; clinical evaluation

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