Mimicking 3D bone microenvironment using a hybrid hydrogel-nanocomposite scaffold and human adipose-derived stem cells for bone differentiation and vascularization

Amel Ibrahim; Oliver FW Gardner; Naira Rodriguez-Florez; John C Hutchinson; Alexander Seifalian; Daniel Thomas-Vazque; Neil J Sebire; David Dunaway; Neil W Bulstrode; Patrizia Ferretti

doi:10.55092/bm20240002

Article

Open Access

Mimicking 3D bone microenvironment using a hybrid hydrogel-nanocomposite scaffold and human adipose-derived stem cells for bone differentiation and vascularization

Amel Ibrahim^1,²^,†^,∗Oliver FW Gardner¹Naira Rodriguez-Florez^1,²^,‡John C Hutchinson³Alexander Seifalian⁴Daniel Thomas-Vazque⁵Neil J Sebire^1,³David Dunaway^1,⁶Neil W Bulstrode^1,²Patrizia Ferretti¹^,†^,∗

¹ Stem Cells and Regenerative Medicine Section, UCL Great Ormond Street Institute of Child Health, London, UK

² Department of Plastic Surgery, Great Ormond Street Hospital for Children NHS Foundation Trust, London, UK

³ Department of Histopathology, Great Ormond Street Hospital for Children, NHS Foundation Trust, London, UK

⁴ Nanotechnology and Regenerative Medicine Commercialization Centre (Ltd), The London Bioscience Innovation Center, London, UK

⁵ Johnson Space Center, NASA Pkwy, Houston, Texas, USA

⁶ Craniofacial Group-FaceValue UCL Great Ormond Street Institute of Child Health and Great Ormond Street Hospital for Children, London, UK

†Current affiliation: Bioworkshop, New York, USA

‡Current affiliation: Universidad de Navarra, TECNUN Escuela de Ingenieros, San Sebastian, Spain and IKERBASQUE, Basque Foundation for Science, Bilbao, Spain

* amel.ibrahim.11@alumni.ucl.ac.uk; p.ferretti@ucl.ac.uk

Volume
Volume 2 Issue 1, 2024
Citation
Ibrahim A, Gardner OF, Rodriguez-Florez N, Hutchinson JC, Seifalian A, et al. Mimicking 3D bone microenvironment using a hybrid hydrogel-nanocomposite scaffold and human adipose-derived stem cells for bone differentiation and vascularization. Biofunct. Mater. 2024(1):0002, https://doi.org/10.55092/bm20240002.
DOI
10.55092/bm20240002
Copyright
Copyright2024 by the authors. Published by ELSP.
Special Issue
Biofunctional Gels

Abstract

Bioengineered bone tissues could bypass limitations of traditional reconstructive methods such as bone grafts or foreign-body implants, but to date this approach has been limited by immaturity of engineered bones and vascularization. Pediatric human adipose-derived stem cells (hADSCs) can be derived using minimally invasive procedures and are known to undergo osteogenic differentiation. Here we have taken a “design-in reverse” approach to tissue engineering by mimicking the native tissue microenvironment to bioengineer complex vascularized tissues. Specifically, we sought to optimize bone tissue engineering by modelling the bone tissue microenvironment and vascularization using hADSCs in combination with a nanocomposite biodegradable nanomaterial and hydrogel, POSS-PCL-fibrin. Our scaffolds exhibited similar architectural features to bone and supported hADSC osteogenic differentiation in vitro. Extensive vascularization and formation of tissues resembling pediatric calvarial (upper part of the skull encasing the brain) bone as well as host cell infiltration yielding chimeric tissues were achieved in vivo in nude mice. Our results suggest a promising dual tissue engineering purpose for POSS-PCL-fibrin both as a scaffold for bioengineering prefabricated replacement bone tissues and potentially as a bioactive scaffold for in situ bone regeneration.

Keywords

Adipose derived stem cells; biomaterial; bone; human; fibrin gel; micro-niche; tissue engineering; vascularization

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